Gold nanoparticle used to replace virus in new CRISPR approach

first_img More information: Kunwoo Lee et al. Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair, Nature Biomedical Engineering (2017). DOI: 10.1038/s41551-017-0137-2AbstractClustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR associated protein 9 (Cas9)-based therapeutics, especially those that can correct gene mutations via homology-directed repair, have the potential to revolutionize the treatment of genetic diseases. However, it is challenging to develop homology-directed repair-based therapeutics because they require the simultaneous in vivo delivery of Cas9 protein, guide RNA and donor DNA. Here, we demonstrate that a delivery vehicle composed of gold nanoparticles conjugated to DNA and complexed with cationic endosomal disruptive polymers can deliver Cas9 ribonucleoprotein and donor DNA into a wide variety of cell types and efficiently correct the DNA mutation that causes Duchenne muscular dystrophy in mice via local injection, with minimal off-target DNA damage. Gene-editing alternative corrects Duchenne muscular dystrophy CRISPR-associated protein Cas9 (white) from Staphylococcus aureus based on Protein Database ID 5AXW. Credit: Thomas Splettstoesser (Wikipedia, CC BY-SA 4.0) CRISPR-Cas9 has been in the news a lot lately because it allows researchers to directly edit genes—either disabling unwanted parts or replacing them altogether. But despite many success stories, the technique still suffers from a major deficit that prevents it from being used as a true medical tool—it sometimes makes mistakes. Those mistakes can cause small or big problems for a host depending on what goes wrong. Prior research has suggested that the majority of mistakes are due to delivery problems, which means that a replacement for the virus part of the technique is required. In this new effort, the researchers report that they have discovered just a such a replacement, and it worked so well that it was able to repair a gene mutation in a Duchenne muscular dystrophy mouse model. The team has named the new technique CRISPR-Gold, because a gold nanoparticle was used to deliver the gene editing molecules instead of a virus.The new package was created by modifying a bit of DNA to cause it to stick to a gold nanoparticle and then a Cas9 protein and also an RNA guide. The package was then coated with a polymer that served as a containment casing—one that also triggered endocytosis (a form of cell transport) and helped the molecules escape endosomes once inside the target cells. The molecules then set to work—the Cas9 cut the target DNA strand, the guide RNA showed what needed to be done and a DNA strand was placed where a mutation had existed. The result was a gene free of a mutation that causes Duchenne muscular dystrophy.There is still a major problem to overcome with the technique, however—it only works in localized applications. Ideally, a mass of packages would be injected into the bloodstream allowing for repair of all cell types, such as muscles impaired by a mutant gene. Explore further (Phys.org)—A team of researchers from the University of California and the University of Tokyo has found a way to use the CRISPR gene editing technique that does not rely on a virus for delivery. In their paper published in the journal Nature Biomedical Engineering, the group describes the new technique, how well it works and improvements that need to be made to make it a viable gene editing tool.center_img Journal information: Nature Biomedical Engineering © 2017 Phys.org Citation: Gold nanoparticle used to replace virus in new CRISPR approach (2017, October 3) retrieved 18 August 2019 from https://phys.org/news/2017-10-gold-nanoparticle-virus-crispr-approach.html This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.last_img read more

Oranje does it at Pragati Maidan

Finally, the grand FDCI-Dutch fashion collaboration saw Dutch designers Jan Taminiau and Diederik Verbakel and Marieke Holthuislabel of DIED team up with our very own Rohit Gandhi and Rahul Khanna of Cue and Suneet Verma. While the collection was interesting, what grabbed eyeballs were interesting headgear, hairdo and killer shoes [by Dutch designer Rosabbe Bergsma] – giving the show a truly international feel. For Taminiau’s collection, the hairdo was done to resemble blooming flowers. For Verma’s show, models walked in wearing feathered headgear. The ensembles marked a fusion between Indian and Dutch sensibilities. read more

The right stand

first_imgBased on women empowerment, an interactive session Ek Nayi Pehchaan was organised in the Capital at Le Meridian on 21 September by AIS Learning. The show was inaugurated by Sharon Lowen, while the guest of honour was Renu Shahnawaz Hussain. The panel of speakers at the programme included – Rina Dhaka, Laxmi Aggarwal, Sanjana Jon, Alka Raghuvanshi, Ramon Lamba and Alexshendra Venus Bakshi.Dhaka spoke on ‘power dressing’ a much talked about concept between women of all ages. Laxshmi Agarwal came up with a new perspective on self-confidence as a tool to overcome all the odds and difficulties of life. Sanjana Jon spoke on ‘celebrating girl child’ and had an interaction with people as how today’s girls face discrimination in many parts of the world simply because of their gender. She holds an aims to create awareness towards improving girl child sex ratio in our country and to ensure that every girl child is given a chance to outshine, by providing her proper education accompanied with basic necessities for her proper growth and development.last_img read more

Music and the classical magic in India

first_imgThe Capital witnessed some of India’s best music traditions on the first two days of the five-day Delhi Classical Music Festival that opened on 4 October at Kamani Auditorium. The opening day of the festival featured the oldest form of Indian classical music – dhrupad. Eminent Dhrupad singers from Madhya Pradesh, Umakant Gundecha and  Ramakant Gundecha, enthralled the audiences with their renditions. The Gundecha brothers were bestowed with the Padmashree award for their contribution to Indian Also Read – ‘Playing Jojo was emotionally exhausting’classical music. Their performance was followed by Hindustani classical music vocalist Pt. Ulhas Kashalkar, a veteran of the Gwalior, Agra and Jaipur, who brought the first evening of the festival to an endearing end. The second evening of the festival saw the performances by two vocalists from two different classical gharanas of north India – Pt. Ajay Pohankar from the Kirana Gharana and Ustad Rashid Khan from the Rampur Sahaswan Gharana. The two vocalists came together to light up the evening with their performances. Also Read – Leslie doing new comedy special with NetflixThe third day marked the aesthetic beauty of thumri and the impeccable taankari of khayal. Renowned veteran of khayal, dhrupad and thumri, 105-year-old Ustad Abdul Rashid Khan, lightened the festival with his renditions. The evening was also shared by Bhuvanesh Komkali, the grandson of late Kumar Gandharva. The upcoming two days at the festival will bring together musicians like –  iconic vocalist Pt Jasraj, sitar maestro Ustad Shahid Parvez, Annupriya Deotale on the violin, Prem Kumar and P Vetri Boopathy on the mridangam and Pt. Narender Nath Dhar, the vocalist of the Etawah Gharana. Being presented by the Department of Art, Culture & Languages, and organised by the Punjabi Academy, the Delhi Classical Music Festival offers an enriching experience of culture to the connoisseurs of art in Delhi. ‘There is immense depth and talent in the field of Hindustani classical music. It is important for us to celebrate India’s classical musical forms and take them to the younger generations. This will keep our art forms alive and encourage the younger generation to absorb and adopt these traditions,’ says S S Yadav, Secretary, Department of Art, Culture & Languages. When: On till 8 October Where: Kamani Auditoriumlast_img read more

Malicious attempts being made to malign Mother Teresa charity CM

first_imgKolkata: Chief Minister Mamata Banerjee launched a scathing attack on the BJP for its “malicious attempts to malign” the name of Missionaries of Charity.This comes after names of two members of Ranchi unit of the Missionaries of Charity have been “dragged” into the controversy involving the sale of babies. As the investigation heats up, Banerjee stepped in to stick up for the Mother Teresa-founded NGO.On Thursday, the Chief Minister, who is at present in North Bengal, tweeted: “Mother Teresa herself set up Missionaries of Charity. And now they are also not being spared. Malicious attempts to malign their name.” Also Read – Speeding Jaguar crashes into Merc, 2 B’deshi bystanders killedRaising her voice against the action, Banerjee further stated in her tweet: “The Sisters are being targeted. #BJP want to spare no one.”She condemned the move and maintained: “Highly condemnable. Let MOC continue to do their work for the poorest of the poor.”The Chief Minister has always raised her voice against “anti-people” moves of the BJP government and over and again, slammed it for its “communal” and at the same time “vendetta” politics.It was on May 22 that Banerjee extended her support for the Archbishop of Delhi, Anil Couto after he wrote a letter stating that “we are witnessing a turbulent political atmosphere which poses a threat to the democratic principles enshrined in our Constitution and the secular fabric of our nation.” Also Read – Naihati: 10 councillors return to TMC from BJPIn this connection, Banerjee had stated: “We have great regard and respect for all communities, caste and creed. We have high regards for all the archbishops of the country. I think it is correct what they have said.”Banerjee also tweeted on Thursday in connection with the Income Tax Department’s raids on Wednesday that were conducted at multiple premises of a hospital group at Rewari in Haryana, which belongs to Swaraj India leader Yogendra Yadav’s sisters and seized cash of around Rs 22 lakh. Banerjee tweeted: “2019 elections are coming. #BJP using the agencies to silence Opposition voices and openly doing political vendetta. Strongly condemn the IT raid on @_YogendraYadav Ji’s family.”It may be recalled that Banerjee had attacked the BJP for its vendetta politics when Trinamool Congress MP Sudip Bandyopadhyay was arrested after he had raised his voice against demonetisation.last_img read more

KIFF to highlight 100 years of Bengali cinema in style

first_imgKolkata: The Kolkata International Film Festival (KIFF) is going to celebrate the 100 years of Bangla Cinema during the 24th KIFF commencing from November 10. Chief Minister Mamata Banerjee will inaugurate the 24th KIFF at Netaji Indoor Stadium on November 10 with superstars like Amitabh Bachchan, Shah Rukh Khan gracing the occasion. The focus country of this year’s festival is Australia and Tunisia will be the “Special Focus” country.On Saturday, chief advisor Aroop Biswas informed that at KIFF, 14 iconic Bengali films will be screened from November 11 to November 17. The celebration will start by screening of Antony Firingee. He also informed that this year, the number of screens have been increased to 16, compared to last year’s 14. “This year, KIFF screening will be available in 16 screens. The PVR Cinema of Howrah and Dum Dum have been included in the list,” said Biswas. Also Read – Rain batters Kolkata, cripples normal lifeDuring the press conference, Minister of State for Information and Cultural Affairs (I&CA) Indranil Sen informed that apart from screening of Bengali films, a film directory will be published during the festival. It will contain information of Bengali films between the years 1917 and 2017. Also a theatre will be staged on the first Bengali filmmaker Hiralal Sen. The act Hiralaler Bioscope will be staged at Madhusudan Mancha on November 15. “Apart from the directory, publishing and staging the act, present day singers will pay their tribute to the legendary singers in the Jalshaghar section. Also a coffee table book will be published in Bengali and English,” said Sen. Also Read – Speeding Jaguar crashes into Mercedes car in Kolkata, 2 pedestrians killedDuring the press conference, actor Prosenjit Chatterjee, Rituparna Sengupta, film director Goutam Ghosh and Vivek Kumar, the Principal Secretary of the I&CA department were also present on the stage. Chatterjee informed that next year, the KIFF will be celebrating its Silver Jubilee. He also said that this year’s film festival is the biggest in KIFF history as the number of films to be screened is the highest, namely 321. “This year’s festival is the trailer. The main film will be shown next year during the Silver Jubilee celebration,” he added.last_img read more

Five ways to lose bat wings

first_imgManage weight, follow a proper skin care regime and eat well to do away with the jiggling of arms, says an expert. Losing weight to keep your body healthy can be a challenge. Even when we start to see the pounds fall off, there are often stubborn spots that seem to want to hold on to extra body fat. Arms are one of those common spots for women. No one likes saggy arms as they make your arms look bigger than they actually are.If your diet is on point and you’re killing it in the gym, but your arms just aren’t where you want them to be, here are some Also Read – Add new books to your shelfsuggestions on how to get rid of bat wings:Exercise: There are many workouts that will help to get fat-free arms. High intensity cardio and interval training exercises help in reduction of bat wings. Some common exercises include push-ups, counter push-ups, scissors and weightlifting.Proper diet: A regular intake of fruits, vegetables and dairy products is a must to maintain a healthy gut that digests the food properly and increases the rate of metabolism. Also Read – Over 2 hours screen time daily will make your kids impulsiveEating healthy is better than eating less and eating food like fried and junk food items. Try to make a diet chart and follow a balanced diet to ward off underarm fat.Weight management: Maintaining an ideal weight is the key. By maintaining a healthy Body Mass Index (BMI), one can avoid the excessive deposition of fat in the body. Diet and exercises play crucial roles in weight management. Food items low in sugar and rich in fibre are must-have in your diet. Processed and refined foods should be avoided to a great extent. Skincare regime: A dry and dehydrated skin highlights your flaws in a big way. Therefore, follow a skin care regime comprising gentle exfoliation and moisturisation so that your arms look good and replenished. A regular massage with aroma oils not only helps you relax, but also tones your arm muscles and helps you get them in shape.Laser treatment: The treatment is fast and lasts for a long time and removes the arm fat under the skin effectively.last_img read more

Learn the art of selfdefence at KCCs Taekwondo classes

first_imgWith the increasing crime rate, Indian parents are looking for a sport for their children that would teach them the art of self-fefence and protection.Korean Cultural Centre India, in view of the same, has organised two months free Taekwondo classes, which will go until October 31.The students will be trained by Master Wan Yong Lee – who will be focusing on teaching self-defense techniques, fights, taekwondo, poomsae, taekwondo demonstration, and dance, teaching etiquettes and manners to the participants. Master Lee has been training Indian students from the age group of six years to 24 years at KCC, which also includes Indian Athletes who participated in this year’s Asian Games.”Unlike the common prejudice about the sport, it is more than a fighting game. Its ultimate goal is to develop and cultivate the personality of the player through physical, mental and spiritual discipline,” he says.Taekwondo – a form of Korean martial art is South Korea’s national sport.It became an official Olympic sport in 2000, and since then it has been disseminated in 203 countries.last_img read more

Better understanding of Indian textiles on a global level

first_imgDesigner Anita Dongre, who has moved one step closer to her dream of empowering the women of India by opening the fourth tailoring unit in the village of Modgaon in Maharashtra, says there has been a sense of urgency within the industry to save the country’s dying crafts. She feels there is a better understanding of Indian textiles and slow fashion on a global level today.”There has been a sense of urgency within the industry to save India’s dying crafts. It’s great to see not just the industry but also the government taking steps towards reviving handloom and making it relevant to the current generation,” said Dongre when asked about her take on how the scene of handloom has changed over the years. Also Read – Add new books to your shelf”Handloom is finally getting its long due recognition but I still think we have a long way to go and a lot more to do,” she added.So where do you see handlooms and textiles moving from here on globally?”There is a better understanding of Indian textiles, crafts and slow fashion, on a global level today. It’s amazing to see the progress we are making towards this as an industry. Social media also has been a huge enabler for us to tell our stories of Indian handlooms and textiles. Also Read – Over 2 hours screen time daily will make your kids impulsiveTalking about the new tailoring unit, the designer said Anita Dongre Foundation works with the local governments and village gram panchayats to create local Women Self Help Groups (SHGs) and bring livelihood or employment opportunities to their own villages. “Empowering the people of these villages by giving them access to a dependable source of income, in their locality is critical to arrest the tide of migration to cities. Modgaon is the fourth location where we have setup a community garment tailoring unit (in addition to Charoti, Jawahar and Dhanevari) in the Palghar District of Maharashtra. “There are no skilled artisans or weavers in these villages – the women from these villages are unskilled labour who we skill in garment tailoring on industrial sewing machines by setting up such training-cum-production centres, and this has been our key objective,” she said.Added Dongre: “During the three months of training period, we train the women in basic and advanced skills of cutting, sewing and finishing garments and during this period every trainee is paid a stipend.””Once the training is complete, we supply the center with fabrics and pay the women for the tailored garments on fair per piece rate basis so as to provide them with regular livelihood opportunities.”last_img read more

OFB generates solar power reduces power intake cost

first_imgKolkata: The Ordnance Factory Board (OFB) has significantly reduced cost of electricity consumption by generating solar power in its various units across the country, an official said here Friday. The OFB is working towards achieving the goal of meeting 45 per cent of its total energy requirements through the solar power route, the official said. The use of solar power has significantly reduced OFB’s cost of electricity consumption from Rs 463.22 lakh during 2015-16 to Rs 163.78 lakh during 2018-19, he said. Also Read – Rs 13,000 crore investment to provide 2 lakh jobs: Mamata”As part of the ongoing efforts to promote ecologically sustainable growth to generate carbon-free clean energy and to reduce expenditure on electricity, the OFB is participating in the National Solar Mission to increase its solar power use to 45 per cent of total consumption,” he said. The installation of grid-connected solar power projects in various OFB units have resulted in generation of approximately 1,47,12,072.14 units of electrical power till November 2018, he said.last_img read more