More information: Kunwoo Lee et al. Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair, Nature Biomedical Engineering (2017). DOI: 10.1038/s41551-017-0137-2AbstractClustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR associated protein 9 (Cas9)-based therapeutics, especially those that can correct gene mutations via homology-directed repair, have the potential to revolutionize the treatment of genetic diseases. However, it is challenging to develop homology-directed repair-based therapeutics because they require the simultaneous in vivo delivery of Cas9 protein, guide RNA and donor DNA. Here, we demonstrate that a delivery vehicle composed of gold nanoparticles conjugated to DNA and complexed with cationic endosomal disruptive polymers can deliver Cas9 ribonucleoprotein and donor DNA into a wide variety of cell types and efficiently correct the DNA mutation that causes Duchenne muscular dystrophy in mice via local injection, with minimal off-target DNA damage. Gene-editing alternative corrects Duchenne muscular dystrophy CRISPR-associated protein Cas9 (white) from Staphylococcus aureus based on Protein Database ID 5AXW. Credit: Thomas Splettstoesser (Wikipedia, CC BY-SA 4.0) CRISPR-Cas9 has been in the news a lot lately because it allows researchers to directly edit genes—either disabling unwanted parts or replacing them altogether. But despite many success stories, the technique still suffers from a major deficit that prevents it from being used as a true medical tool—it sometimes makes mistakes. Those mistakes can cause small or big problems for a host depending on what goes wrong. Prior research has suggested that the majority of mistakes are due to delivery problems, which means that a replacement for the virus part of the technique is required. In this new effort, the researchers report that they have discovered just a such a replacement, and it worked so well that it was able to repair a gene mutation in a Duchenne muscular dystrophy mouse model. The team has named the new technique CRISPR-Gold, because a gold nanoparticle was used to deliver the gene editing molecules instead of a virus.The new package was created by modifying a bit of DNA to cause it to stick to a gold nanoparticle and then a Cas9 protein and also an RNA guide. The package was then coated with a polymer that served as a containment casing—one that also triggered endocytosis (a form of cell transport) and helped the molecules escape endosomes once inside the target cells. The molecules then set to work—the Cas9 cut the target DNA strand, the guide RNA showed what needed to be done and a DNA strand was placed where a mutation had existed. The result was a gene free of a mutation that causes Duchenne muscular dystrophy.There is still a major problem to overcome with the technique, however—it only works in localized applications. Ideally, a mass of packages would be injected into the bloodstream allowing for repair of all cell types, such as muscles impaired by a mutant gene. Explore further (Phys.org)—A team of researchers from the University of California and the University of Tokyo has found a way to use the CRISPR gene editing technique that does not rely on a virus for delivery. In their paper published in the journal Nature Biomedical Engineering, the group describes the new technique, how well it works and improvements that need to be made to make it a viable gene editing tool. Journal information: Nature Biomedical Engineering © 2017 Phys.org Citation: Gold nanoparticle used to replace virus in new CRISPR approach (2017, October 3) retrieved 18 August 2019 from https://phys.org/news/2017-10-gold-nanoparticle-virus-crispr-approach.html This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
Finally, the grand FDCI-Dutch fashion collaboration saw Dutch designers Jan Taminiau and Diederik Verbakel and Marieke Holthuislabel of DIED team up with our very own Rohit Gandhi and Rahul Khanna of Cue and Suneet Verma. While the collection was interesting, what grabbed eyeballs were interesting headgear, hairdo and killer shoes [by Dutch designer Rosabbe Bergsma] – giving the show a truly international feel. For Taminiau’s collection, the hairdo was done to resemble blooming flowers. For Verma’s show, models walked in wearing feathered headgear. The ensembles marked a fusion between Indian and Dutch sensibilities.
Based on women empowerment, an interactive session Ek Nayi Pehchaan was organised in the Capital at Le Meridian on 21 September by AIS Learning. The show was inaugurated by Sharon Lowen, while the guest of honour was Renu Shahnawaz Hussain. The panel of speakers at the programme included – Rina Dhaka, Laxmi Aggarwal, Sanjana Jon, Alka Raghuvanshi, Ramon Lamba and Alexshendra Venus Bakshi.Dhaka spoke on ‘power dressing’ a much talked about concept between women of all ages. Laxshmi Agarwal came up with a new perspective on self-confidence as a tool to overcome all the odds and difficulties of life. Sanjana Jon spoke on ‘celebrating girl child’ and had an interaction with people as how today’s girls face discrimination in many parts of the world simply because of their gender. She holds an aims to create awareness towards improving girl child sex ratio in our country and to ensure that every girl child is given a chance to outshine, by providing her proper education accompanied with basic necessities for her proper growth and development.
The Capital witnessed some of India’s best music traditions on the first two days of the five-day Delhi Classical Music Festival that opened on 4 October at Kamani Auditorium. The opening day of the festival featured the oldest form of Indian classical music – dhrupad. Eminent Dhrupad singers from Madhya Pradesh, Umakant Gundecha and Ramakant Gundecha, enthralled the audiences with their renditions. The Gundecha brothers were bestowed with the Padmashree award for their contribution to Indian Also Read – ‘Playing Jojo was emotionally exhausting’classical music. Their performance was followed by Hindustani classical music vocalist Pt. Ulhas Kashalkar, a veteran of the Gwalior, Agra and Jaipur, who brought the first evening of the festival to an endearing end. The second evening of the festival saw the performances by two vocalists from two different classical gharanas of north India – Pt. Ajay Pohankar from the Kirana Gharana and Ustad Rashid Khan from the Rampur Sahaswan Gharana. The two vocalists came together to light up the evening with their performances. Also Read – Leslie doing new comedy special with NetflixThe third day marked the aesthetic beauty of thumri and the impeccable taankari of khayal. Renowned veteran of khayal, dhrupad and thumri, 105-year-old Ustad Abdul Rashid Khan, lightened the festival with his renditions. The evening was also shared by Bhuvanesh Komkali, the grandson of late Kumar Gandharva. The upcoming two days at the festival will bring together musicians like – iconic vocalist Pt Jasraj, sitar maestro Ustad Shahid Parvez, Annupriya Deotale on the violin, Prem Kumar and P Vetri Boopathy on the mridangam and Pt. Narender Nath Dhar, the vocalist of the Etawah Gharana. Being presented by the Department of Art, Culture & Languages, and organised by the Punjabi Academy, the Delhi Classical Music Festival offers an enriching experience of culture to the connoisseurs of art in Delhi. ‘There is immense depth and talent in the field of Hindustani classical music. It is important for us to celebrate India’s classical musical forms and take them to the younger generations. This will keep our art forms alive and encourage the younger generation to absorb and adopt these traditions,’ says S S Yadav, Secretary, Department of Art, Culture & Languages. When: On till 8 October Where: Kamani Auditorium
Kolkata: Chief Minister Mamata Banerjee launched a scathing attack on the BJP for its “malicious attempts to malign” the name of Missionaries of Charity.This comes after names of two members of Ranchi unit of the Missionaries of Charity have been “dragged” into the controversy involving the sale of babies. As the investigation heats up, Banerjee stepped in to stick up for the Mother Teresa-founded NGO.On Thursday, the Chief Minister, who is at present in North Bengal, tweeted: “Mother Teresa herself set up Missionaries of Charity. And now they are also not being spared. Malicious attempts to malign their name.” Also Read – Speeding Jaguar crashes into Merc, 2 B’deshi bystanders killedRaising her voice against the action, Banerjee further stated in her tweet: “The Sisters are being targeted. #BJP want to spare no one.”She condemned the move and maintained: “Highly condemnable. Let MOC continue to do their work for the poorest of the poor.”The Chief Minister has always raised her voice against “anti-people” moves of the BJP government and over and again, slammed it for its “communal” and at the same time “vendetta” politics.It was on May 22 that Banerjee extended her support for the Archbishop of Delhi, Anil Couto after he wrote a letter stating that “we are witnessing a turbulent political atmosphere which poses a threat to the democratic principles enshrined in our Constitution and the secular fabric of our nation.” Also Read – Naihati: 10 councillors return to TMC from BJPIn this connection, Banerjee had stated: “We have great regard and respect for all communities, caste and creed. We have high regards for all the archbishops of the country. I think it is correct what they have said.”Banerjee also tweeted on Thursday in connection with the Income Tax Department’s raids on Wednesday that were conducted at multiple premises of a hospital group at Rewari in Haryana, which belongs to Swaraj India leader Yogendra Yadav’s sisters and seized cash of around Rs 22 lakh. Banerjee tweeted: “2019 elections are coming. #BJP using the agencies to silence Opposition voices and openly doing political vendetta. Strongly condemn the IT raid on @_YogendraYadav Ji’s family.”It may be recalled that Banerjee had attacked the BJP for its vendetta politics when Trinamool Congress MP Sudip Bandyopadhyay was arrested after he had raised his voice against demonetisation.
Kolkata: The Kolkata International Film Festival (KIFF) is going to celebrate the 100 years of Bangla Cinema during the 24th KIFF commencing from November 10. Chief Minister Mamata Banerjee will inaugurate the 24th KIFF at Netaji Indoor Stadium on November 10 with superstars like Amitabh Bachchan, Shah Rukh Khan gracing the occasion. The focus country of this year’s festival is Australia and Tunisia will be the “Special Focus” country.On Saturday, chief advisor Aroop Biswas informed that at KIFF, 14 iconic Bengali films will be screened from November 11 to November 17. The celebration will start by screening of Antony Firingee. He also informed that this year, the number of screens have been increased to 16, compared to last year’s 14. “This year, KIFF screening will be available in 16 screens. The PVR Cinema of Howrah and Dum Dum have been included in the list,” said Biswas. Also Read – Rain batters Kolkata, cripples normal lifeDuring the press conference, Minister of State for Information and Cultural Affairs (I&CA) Indranil Sen informed that apart from screening of Bengali films, a film directory will be published during the festival. It will contain information of Bengali films between the years 1917 and 2017. Also a theatre will be staged on the first Bengali filmmaker Hiralal Sen. The act Hiralaler Bioscope will be staged at Madhusudan Mancha on November 15. “Apart from the directory, publishing and staging the act, present day singers will pay their tribute to the legendary singers in the Jalshaghar section. Also a coffee table book will be published in Bengali and English,” said Sen. Also Read – Speeding Jaguar crashes into Mercedes car in Kolkata, 2 pedestrians killedDuring the press conference, actor Prosenjit Chatterjee, Rituparna Sengupta, film director Goutam Ghosh and Vivek Kumar, the Principal Secretary of the I&CA department were also present on the stage. Chatterjee informed that next year, the KIFF will be celebrating its Silver Jubilee. He also said that this year’s film festival is the biggest in KIFF history as the number of films to be screened is the highest, namely 321. “This year’s festival is the trailer. The main film will be shown next year during the Silver Jubilee celebration,” he added.
Manage weight, follow a proper skin care regime and eat well to do away with the jiggling of arms, says an expert. Losing weight to keep your body healthy can be a challenge. Even when we start to see the pounds fall off, there are often stubborn spots that seem to want to hold on to extra body fat. Arms are one of those common spots for women. No one likes saggy arms as they make your arms look bigger than they actually are.If your diet is on point and you’re killing it in the gym, but your arms just aren’t where you want them to be, here are some Also Read – Add new books to your shelfsuggestions on how to get rid of bat wings:Exercise: There are many workouts that will help to get fat-free arms. High intensity cardio and interval training exercises help in reduction of bat wings. Some common exercises include push-ups, counter push-ups, scissors and weightlifting.Proper diet: A regular intake of fruits, vegetables and dairy products is a must to maintain a healthy gut that digests the food properly and increases the rate of metabolism. Also Read – Over 2 hours screen time daily will make your kids impulsiveEating healthy is better than eating less and eating food like fried and junk food items. Try to make a diet chart and follow a balanced diet to ward off underarm fat.Weight management: Maintaining an ideal weight is the key. By maintaining a healthy Body Mass Index (BMI), one can avoid the excessive deposition of fat in the body. Diet and exercises play crucial roles in weight management. Food items low in sugar and rich in fibre are must-have in your diet. Processed and refined foods should be avoided to a great extent. Skincare regime: A dry and dehydrated skin highlights your flaws in a big way. Therefore, follow a skin care regime comprising gentle exfoliation and moisturisation so that your arms look good and replenished. A regular massage with aroma oils not only helps you relax, but also tones your arm muscles and helps you get them in shape.Laser treatment: The treatment is fast and lasts for a long time and removes the arm fat under the skin effectively.
With the increasing crime rate, Indian parents are looking for a sport for their children that would teach them the art of self-fefence and protection.Korean Cultural Centre India, in view of the same, has organised two months free Taekwondo classes, which will go until October 31.The students will be trained by Master Wan Yong Lee – who will be focusing on teaching self-defense techniques, fights, taekwondo, poomsae, taekwondo demonstration, and dance, teaching etiquettes and manners to the participants. Master Lee has been training Indian students from the age group of six years to 24 years at KCC, which also includes Indian Athletes who participated in this year’s Asian Games.”Unlike the common prejudice about the sport, it is more than a fighting game. Its ultimate goal is to develop and cultivate the personality of the player through physical, mental and spiritual discipline,” he says.Taekwondo – a form of Korean martial art is South Korea’s national sport.It became an official Olympic sport in 2000, and since then it has been disseminated in 203 countries.
Designer Anita Dongre, who has moved one step closer to her dream of empowering the women of India by opening the fourth tailoring unit in the village of Modgaon in Maharashtra, says there has been a sense of urgency within the industry to save the country’s dying crafts. She feels there is a better understanding of Indian textiles and slow fashion on a global level today.”There has been a sense of urgency within the industry to save India’s dying crafts. It’s great to see not just the industry but also the government taking steps towards reviving handloom and making it relevant to the current generation,” said Dongre when asked about her take on how the scene of handloom has changed over the years. Also Read – Add new books to your shelf”Handloom is finally getting its long due recognition but I still think we have a long way to go and a lot more to do,” she added.So where do you see handlooms and textiles moving from here on globally?”There is a better understanding of Indian textiles, crafts and slow fashion, on a global level today. It’s amazing to see the progress we are making towards this as an industry. Social media also has been a huge enabler for us to tell our stories of Indian handlooms and textiles. Also Read – Over 2 hours screen time daily will make your kids impulsiveTalking about the new tailoring unit, the designer said Anita Dongre Foundation works with the local governments and village gram panchayats to create local Women Self Help Groups (SHGs) and bring livelihood or employment opportunities to their own villages. “Empowering the people of these villages by giving them access to a dependable source of income, in their locality is critical to arrest the tide of migration to cities. Modgaon is the fourth location where we have setup a community garment tailoring unit (in addition to Charoti, Jawahar and Dhanevari) in the Palghar District of Maharashtra. “There are no skilled artisans or weavers in these villages – the women from these villages are unskilled labour who we skill in garment tailoring on industrial sewing machines by setting up such training-cum-production centres, and this has been our key objective,” she said.Added Dongre: “During the three months of training period, we train the women in basic and advanced skills of cutting, sewing and finishing garments and during this period every trainee is paid a stipend.””Once the training is complete, we supply the center with fabrics and pay the women for the tailored garments on fair per piece rate basis so as to provide them with regular livelihood opportunities.”
Kolkata: The Ordnance Factory Board (OFB) has significantly reduced cost of electricity consumption by generating solar power in its various units across the country, an official said here Friday. The OFB is working towards achieving the goal of meeting 45 per cent of its total energy requirements through the solar power route, the official said. The use of solar power has significantly reduced OFB’s cost of electricity consumption from Rs 463.22 lakh during 2015-16 to Rs 163.78 lakh during 2018-19, he said. Also Read – Rs 13,000 crore investment to provide 2 lakh jobs: Mamata”As part of the ongoing efforts to promote ecologically sustainable growth to generate carbon-free clean energy and to reduce expenditure on electricity, the OFB is participating in the National Solar Mission to increase its solar power use to 45 per cent of total consumption,” he said. The installation of grid-connected solar power projects in various OFB units have resulted in generation of approximately 1,47,12,072.14 units of electrical power till November 2018, he said.
Growing up in a secular neighbourhood, I always saw Holi as an occasion to celebrate togetherness – ignoring the existing societal barriers, cultural differences, and religious norms. However, time changed everything.Gathering in the community park to splash colours on anyone passing by, singing and dancing to the tunes of holi-centric songs, and binge eating delicacies until the stomach starts to ache, all of it has become a rare sight nowadays. Also Read – Add new books to your shelfLiquor has replaced the traditional Bhaang, and mouthwatering sweets (including Gujiya and jalebi) are avoided out of diet concerns. Overall, the festival of colours has reduced to ‘a day off’ from office. Contemplating the present situation, commoners talk about how the way of celebrating Holi has changed over the years. In search of a better standard of living, many people have migrated from villages to cities – and in the process, self-centralism has become a predominant force. Also Read – Over 2 hours screen time daily will make your kids impulsiveAccording to various psychological studies, stress levels are also on the rise which has resulted in the decline of patience levels and people tend to be ‘intolerant’ less bothered about others. -Harsh Khanna, Businessman Until a decade ago, we actually used to gather at the community centre of our residential society and celebrate the festival. It used to be hours of celebration with neighbours, relatives, and a thousand friends. But the increasing economic burden on families has forced people to keep the celebrations minimal. Instead of large gatherings, it is just about close friends and relatives. Moreover, urbanisation and rise of nuclear families has further worsen the situation. -Mayank Bhardwaj, Engineer Why just Holi? Look at any festival that we celebrate. We’re more virtually connected to each other now, and the physical connect is almost non-existent. Also, we are no longer interested in nurturing relationships and rather prefer spending ‘me time’ – binge-watching the trending shows. The charm, and spirit of festivals have almost disappeared, and they are losing the real essence. -Akshit Juneja, Entrepreneur I believe the community culture changes after every 100 kilometers. Owing to the different lifestyles and working patterns, way of celebrating an occasion also differs a lot. It was only after I migrated to a metro city that I observed how people in rural areas are more sociable, and stressful lives of city dwellers hampers the festive joy. Having said that, I don’t believe the community culture has completely lost its existence today. It’s still extant. Even today, people like me celebrate the occasion with family, neighbours, and cousins. The only difference that I observe is the amount of enthusiasm and zest, which has dropped down with age. And I believe it is very natural. -Akshay Kumar, Pr Professional I enjoyed Holi celebration more as a kid than I do now. Today, it feels unsafe to step out of the house and roam around the society freely.Earlier the preparations would begin at least a week before – where we would fill buckets with water balloons, stock up colours boxes, look out for the coolest pichkaris in the market, and play for hours without worrying about anything; but things have changed to a great extent. Now, Holi celebration is more of a custom which is mandatorily practiced, but wraps up within a few hours.-Gayatri Mohan, Journalist
A champion is someone who certainly exceeds expectations–a person of tremendous motivation striving for victory. The term applies not only to sports but also to fields such as science, human rights, and politics. There are many champions in the history of civilization. Then there’s Shavarsh Karapetyan.Even though this lavish introduction sounds a tad exaggerated, bear with me, for when you learn about this man’s achievements, you will certainly agree that he deserves it.Retired finswimmer Shavarsh Karapetyan attends the opening ceremony of the 2nd International Motivational Film Festival ‘Bridge of Arts.’ Valery Matytsin/TASS (Photo by Valery Matytsin\TASS via Getty Images)After all, Karapetyan, an Armenian-born Soviet finswimmer, won the world championship 17 times. He is also a 13-time European champion, and a seven-time champion of his homeland, the USSR. Apart from this, Karapetyan broke the World Record 11 times. One might say that he is the embodiment of finswimming itself.Yerevan, Armenia.However, what makes Karapetyan more than a champion fin swimmer are not the medals on his wall, but his relentless sacrifice for others. In 1976, he personally saved 20 people from drowning after a trolley bus flew off the road and into a frozen lake near Yerevan, the capital of Armenia.But in order to understand his act of courage, we first need to take a peek into his life and career. Perhaps the crucial experience that led Karapetyan to turn to professional swimming was his narrow escape from death as a 15-year-old. He was beaten up by a group of hoodlums who tied him to a rock and threw him into a nearby lake.Swan Lake in Yerevan, Armenia.By strength alone, he managed to tear the ropes off his hands, liberate himself from the stone that was dragging him to the bottom, and swim out victorious. After this incident, he took up swimming, but due to petty rivalry was denied the right to compete on his national team.Pilot Becomes A Hero During This Inspiring SituationSo he switched to finswimming and quickly rose to prominence, winning a number of state-level competitions. By the age of 18, he was already the champion of the Soviet Union and just two months later, he became the European champion by breaking the World Record.Finswimming relay medals podium at World Games I in 1981. Photo by Jeff In Ca CC BY-SA 4.0Envy followed him wherever he went, and there was even an attempt on his life by a fellow competitor who sabotaged his oxygen tank during a championship in Kiev. Even with this handicap, which nearly cost him his life, Karapetyan won the race.He had another brush with death in 1974, when a bus he was riding on almost fell off a cliff. In the midst of panic, the swimmer took the steering wheel and carefully rode the bus into reverse, until reaching safety. Thirty lives, including his own, were saved on that day, thanks to his initiative.Old photo of Yerevan, Armenia, c. 1963.But what followed defined the rest of his life. Two years after this incident, Karapetyan was conducting his usual morning exercise of running beside Yerevan Lake when a trolleybus hurtled past him and fell right into the frozen reservoir.Due to the sheer power of the impact, most of the 92 passengers aboard lost consciousness, while their transport-turned-death trap was sinking to the bottom of the freezing lake. Without hesitation, the professional diver knew what to do. He jumped into the water, broke the glass window of the trolleybus with his bare feet, and started pulling the people out.Shavarsh Karapetyan 2014. Photo by Канал Люди CC BY 3.0The bus settled on the lake bed, 33 feet underwater and 80 feet from the shore, and Karapetyan had to make 30 consecutive dives in order to pull out as many people as he could. In the end, 20 of the passengers were saved. He managed to pull out more, but for some, it was already too late.Even though the incident was a complete disaster, if it weren’t for his heroism, it would have been much worse. As for Karapetyan, the price was high. After his 30th dive, he lost consciousness himself, as a result of a lack of oxygen.Finswimmer. Photo by Cimba69 CC BY SA 4.0Later, the consequences of such a superhuman effort took hold─the swimmer was diagnosed with pneumonia and blood contamination from the polluted industrial water.After a 46-day coma, the hero of Yerevan Lake finally woke up. Still, his career was over due to the extreme nervous exhaustion which took hold.Even though one would expect that instant recognition was the least he deserved, the story of his heroism remained largely unknown until 1982, when an article was published in the state-wide newspaper Komsomolskaya Pravda, to commemorate his extraordinary feat.Yerevan Lake. Photo by I, Bouarf CC BY-SA 3.0Until then, the fact that it was the champion himself who saved all those people was known only to the locals of Yerevan. After the article titled The Underwater Battle of the Champion, Karapetyan received 75,000 letters of praise and thanks and became a household name in the USSR.Retired Soviet-Armenian finswimmer Shavarsh Karapetyan. (Photo by Artyom Geodakyan\TASS via Getty Images)Modest by nature, he never saw it as heroism. Rather, he was frustrated by the fact that so many others died in the crash that day. When asked in an interview about the event, he replied:“I knew that I could only save so many lives, I was afraid to make a mistake. It was so dark down there that I could barely see anything. On one of my dives, I accidentally grabbed a seat instead of a passenger… I could have saved a life instead. That seat still haunts me in my nightmares.”Since then Shavarsh Karapetyan had been celebrated and awarded a number of times, including two medals bestowed upon him by his own government, a UNESCO “Fair Play” award, and the honor of having an asteroid named after him: 3027 Shavarsh.Related Article: The “Bad” from Good, Bad and the Ugly was a WW2 HeroIn 2014 he carried the torch for the Winter Olympics in Moscow, where he currently resides as the owner of a shoe shop called The Second Breath.Karapetyan leads a quiet life below the radar, but the memory of his heroism remains as vivid as ever, for people never forget a champion.Nikola Budanovic is a freelance journalist who has worked for various media outlets such as Vice, War History Online, The Vintage News, and Taste of Cinema. His areas of interest include history, particularly military history, literature and film.
RMS Queen Elizabeth was an ocean liner operated by Cunard Line and contracted to carry Royal Mail. She and her sister RMS Queen Mary were the pride of Cunard Line as they easily adapted to the ever-changing shipping market. They endured World War II as warships and lasted until the end of the Age of Ocean Liners, when travel moved from the waters to the air. After being retired, RMS Queen Mary now rests in a California harbor and serves as a floating hotel. Unfortunately, her sister the Queen Elizabeth suffered a great disaster. She burned in a fire and sank in Hong Kong harbor while undergoing refurbishment. The cause of the fire has never been officially determined.RMS Queen Elizabeth entering New York harbor in 1965, taken from the boat deck, early morning. Photo by Jongleur100 CC BY 3.0The story of this great ship begins in the 1930s when it was constructed by John Brown & Company in Clydebank, Scotland. At the time of the construction she was known as Hull 552 and later, when work was done, she was one of the most elegant ships and the largest passenger liner ever constructed (1,031 feet long, and 118.5 feet wide), and named in honor of Queen Elizabeth I.Along with her sister-vessel, she was launched on September 27, 1938, but the outbreak of World War II prevented their maiden voyage as passenger liners until 1946.Digitally altered depiction of the RMS Queen Elizabeth, based on a free-use photo of the RMS Queen Mary.RMS Elizabeth finally set sail on November 13, 1940, when she was requisitioned by the Ministry of Transport. She sailed to Singapore where she got converted into a troopship, outfitted with anti-aircraft guns and enough bunks to carry 5,000 people.It is said that during the war, RMS Queen Elizabeth carried nearly 1 million soldiers and sailed over 500,000 miles. After the end of World War II, the vessel was brought back to the shipyard to be converted back into a civilian liner and was finally returned to commercial service.RMS Queen Elizabeth at Southampton, c. 1968 (exact date unknown). Photo by Lindemann97 – CC BY-SA 4.0She left on her maiden passenger voyage in October 1946, and in the following 20 years, RMS Queen Elizabeth was one of the dominant transatlantic carriers. However, in the 1960s air travel became rapidly popular, and many ships lost their importance in transport.Canadian troops leaving Europe, December 1945, sailing home on board the RMS Queen Elizabeth.Cunard planned to operate the RMS Queen Elizabeth on Bahaman cruises and in 1965, she underwent a refit suitable for the cruise market. An outdoor swimming pool, lido deck, and an enhanced air conditioning were added. But things didn’t go as expected.It turned out that RMS Queen Elizabeth was too uneconomical to fuel while her size was too large to use the Panama Canal as well as most of the ports in the region.The Cunard flagship and world’s largest liner RMS Queen Elizabeth, afloat and apparently with auxiliary power, in the King George V dry dock. There is little room to spare. Photo By Ian Taylor CC BY 2.0On May 8, 1967, it was announced that both Queens were to be retired, and on November 5, 1968 RMS Queen Elizabeth crossed the Atlantic for the last time before being sold to The Queen Corporation company in Philadelphia.The new owners turned her into a floating hotel but only after two years, she suffered a fire and the local authorities closed her down.In 1970, RMS Queen Elizabeth was up for sale again and was sold at an auction to a Hong Kong businessman named Tung Chao Yung, the head of the Orient Overseas Line, who envisioned converting the ship into a floating university.Tung Chao Yung renamed the ship as Seawise University and she was sent to Hong Kong, where her original interiors were removed and new machinery was added.RMS Queen Elizabeth in Cherbourg (Normandy, France) in 1966. Photo Queen_Elizabethaa CC BY SA 3.0Before being completed, on January 9, 1972, five separate fires broke out. Despite the efforts of the fireboats that worked hard for 24 hours, the fire spread everywhere and it completely destroyed the ship.The cause of the fires was never explained which left space for rumors. Many believed that the fires were set deliberately as part of an insurance fraud since Tung Chao Yung insured the ship for $8 million, while it cost him only $3.5 million to buy it.All that was left of the elegant RMS Queen Elizabeth was a half sunken hulk of twisted, melted steel that sat rusting in the harbor until 1990 when it was finally buried in millions of tons of rock and concrete.Read another story from us: Early-Christian Basilica Found at the Bottom of a Lake could be Momentous SiteBefore being buried, the wreck of the ship featured as the MI6 Headquarters in the James Bond film The Man with Golden Gun. The shipwreck of RMS Queen Elizabeth was the largest one until the Costa Concordia disaster in 2012.
Castles are typically associated with acts of war. Impenetrable fortresses which echo with the sound of battles past, their stones stained with blood. This isn’t the case with Himeji Castle in Japan. The structure has certainly been involved in its fair share of chaos and political intrigue. Yet this impressively preserved remnant of feudal society represents peace as well as conflict.Himeji, Japan – The main keep of Himeji Castle. Founded in 1333 and rebuilt in the early 1600’s, the castle is considered one of the best preserved in Japan.Overlooking the city of Himeji from the vantage point of the Himeyama hilltop, it has dominated the skyline in various forms since 1346.Other names for it include Shirasagijo, or the “White Egret” castle, due to its dazzling exterior and wing-like architecture. The bird comparison also led to it being called Hakurojo, the “White Heron.”The castle’s main support comprises a surprisingly simple arrangement, fashioned out of wood.A depiction of the intricate castle complexA Telegraph piece from 2015 described these main pillars as “a pair of gigantic chopsticks 87ft high, tapering from a diameter of around 38in at their base.” One is made of fir and the other cypress.Himeji Castle is still standing thanks to these so-called sticks plus “some clever joinery and a lick of plaster,” though a lot of work has been done on the property over the centuries. As might be expected, it is classed as a World Heritage site.A 1761 depiction of the castle complexThe fact it stands at all is a historic miracle. Amazingly the castle has survived not only the bombing of Himeji during World War II (it was seen as a key strategic location by the Americans) but also a major natural disaster, the Great Hanshin earthquake of 1995.When building commenced on the hill in 1333, it was actually for a fort which wound up being demolished 13 years later. The beginnings of the castle were the brainchild of Akamatsu Sadanori, son of Akamatsu Norimura, samurai and governor of Harima Province. Himeji is found in the middle of this Province.Himeji Castle in the spring of 2009Over the next couple of centuries, the place saw some changes, not only in people but also structure. At first it was named Himeyama Castle, which was changed to Himeji Castle during the rule of Kuroda Shigetaka in the mid-16th century.He remodeled the site, but it was only the tip of the iceberg when it came to home improvements. A keep was erected, then knocked down. The early 17th century saw the area developed into a complex resembling what tourists see today.Weapon racks inside the keep Photo by Corpse Reviver CC BY 3.0This was the idea of Ikeda Terumasa, who had three moats added, amongst other developments. His father in law, Tokugawa Ieyasu, was the previous owner and a renowned warrior-statesman.He viewed Himeji Castle as a formidable symbol that would deter violence rather than encourage it. Ieyasu was considered a great unifying force in Japanese culture.Ikeda TerumasaIn a CNN interview from 2018, Dr. Jennifer Mitchelhill (author of Samurai Castles) explained that “the samurai were not just warriors. They were also castle-builders, politicians and local leaders with a great aesthetic sense.”Ieyasu gave Himeji Castle to Ikeda Terumasa as thanks for his help during the recently-concluded Battle of Sekigahara. The structure became an intricate series of over 80 buildings designed to confuse invaders.Grave of Ikeda Terumasa Photo by Reggaeman CC BY SA 3.0Visitors to the castle face a challenge navigating the complex, and have to follow floor markers so they don’t get lost.A tour guide is quoted as saying, “The layout is ingenious, with spiralling paths, blind corners, hidden hatches and “trick” gates which could be entirely blocked with rubble within seconds.”Another unusual feature of castle defences was the policy of pouring boiling water on an enemy instead of oil. This was to avoid staining the plaster work.Himeji, Japan – April 3, 2016: Himeji Castle with beautiful cherry blossom in spring season. It is regarded as the finest surviving example of prototypical Japanese castle architectureHimeji Castle isn’t a typical tourist attraction. According to the Telegraph “the ninja spirit of concealment and stealth is evoked in the design of the castle itself.” A troop of ninja even lived in the grounds.Despite these intimidating details, the most famous battle fought at Himeji was a fictional one. The 1967 James Bond film You Only Live Twice used the castle as the setting for Sean Connery’s ninja training sequences.Himeji, Japan – October 24th, 2014: Autumn had arrived to Himeji-jo Castle. Himeji Castle is a hilltop Japanese castle complex located in Himeji, in Hy?go Prefecture, Japan. The castle is regarded as the finest surviving example of prototypical Japanese castle architecture, comprising a network of 83 buildings with advanced defensive systems from the feudal period.Like any ancient building, Himeji Castle has its ghosts. It’s famous for inspiring one of the most frightening horror movies of recent years, The Ring (Ringu), via its tale of a spurned samurai and his brutal revenge on the object of his affection.Tessan Aoyama was the woo’ing warrior, with his sights set on servant Okiku. However when she rejected him he devised a fiendish scheme to force her into a union.Himeji, Japan – May 16, 2015: Main tower of the Himeji Castle, the white Heron castle, Japan. UNESCO world heritage site after restauration and reopening.A 2017 article on the horror website Bloody Disgusting tells that “One of Okiku’s primary duties was to look after ten highly valuable golden plates that were owned by Aoyama, and one day, the samurai decided to hide one of them. He told Okiku that if she didn’t agree to be with him, he would blame her for stealing the plate, which would lead to her torture and execution.”A charming character indeed! When that plan failed he killed her by throwing her down a well. This can be found outside the castle and is known as “Okiku’s Well.” Her ghost was said to emerge on all fours out of the well to torment Aoyama, who then lost his mind.From this, the iconic black-haired Sadako/Samara was born to a terrified moviegoing public.Himeji Castle has stood for hundreds of years, withstanding not only the test of time but the ravages of history.Read another story from us: Ancient Italian ‘Castle in the Sky’ in Breathtaking Location Now Available to RentIt remains a popular destination for culture buffs and ghost hunters alike. Will it still be there in another few centuries? On this evidence, the signs are highly encouraging.
Brees will move ahead of Rodgers in the all-time QB rankings with another Super BowlColin thinks Drew Brees and the Saints have a legitimate shot to win the Super Bowl, and believes that another title would move Brees past Aaron Rodgers in the all-time rankings of NFL quarterbacks. Even though Rodgers is largely seen as the poster child for a quarterback carrying an organization, Brees has had to overcome constant challenges in New Orleans, including Bountygate and the aftermath of Katrina.Most consider Rodgers ahead of Brees at this point, but another title would give Brees the edge in the all-time rankings, in Colin’s eyes. Alex Guerrero will be the scapegoat if the Pats don’t win it allThe Patriots will play their first game since the bombshell article revealed lingering tensions in the organization, and Colin thinks Alex Guerrero will be the scapegoat if New England can’t win another Super Bowl.Spygate and Deflategate both saw low level employees take the fall for the Pats, even though they were clearly taking orders, but in this case, Guerrero is the easiest person to point to for creating division between Brady and Belichick, even if it’s not entirely his fault.Guests: Nick Wright – Host of First Thing’s First is in-studio explaining why LeBron hasn’t made his decision yet; and defending Cam Newton.Chris Simms – Former NFL QB joins the show to talk Gruden to the Raiders; his experience playing for Gruden; and his take on Patriots drama from his time there.Michael Rappaport – Actor/Comedian and host of the I Am Rappaport Stereo Podcast is in-studio why Luke Walton and the Lakers need to fight fire with fire to end their LaVar Ball problem; why he would trade Lonzo; and why he doesn’t think LeBron has made a free agency decision, yet.Ray Lewis – FS1 NFL Analyst and former Ravens All-Pro is in-studio discussing his doubts about Gruden to the Raiders; and how the Lakers are mishandling the LaVar Ball distraction.
Advertisement Episode 12The NFL Draft is a wrap and Middlekauff gives his best and worst picks from the first round; explains why the Browns could ruin Baker Mayfield’s career; and why Saquon Barkley was a massive mistake for the Giants at 2 in a running back heavy draft.John also looks at the power struggle in Oakland between Jon Gruden and GM Reggie McKenzie and why Gruden’s questionable draft shows why he needs to hire a personnel guy to keep him in check. Follow John on Twitter at @JohnMiddlekauff and download and subscribe to the 3 and Out on Apple, iHeart or wherever you download your podcasts now!
Also: – Colin’s Top 5 NFL coaches on the hot seat– Colin’s Week 1 CFB SpoilersGuests:Al Michaels – SNF Announcer and 5-time Emmy Winner; Ryan Leaf – Former All-American and NFL QB; Greg Cosell – NFL Films Senior Producer; Bucky Brooks – NFL Network Analyst and former scout. Baker Mayfield will be the Browns’ starter before people thinkBaker Mayfield got his first start for the Browns in Cleveland’s final preseason game against the Lions, and was impressive completing 9-of-16 passes for 138 yards while leading three scoring drives, albeit against a Detroit team that sat all of its starters.Colin doesn’t think there is much to takeaway from the final preseason game, but does think Mayfield’s performance will stick in the minds of Cleveland fans, and management. When Tyrod Taylor inevitably struggles at some point this year, fans last memory of Baker will be of him shredding the Lions, which will lead to a quick hook for Taylor and Mayfield starting sooner than most people are predicting.
October 1, 2005 This story appears in the October 2005 issue of Entrepreneur. Subscribe » 2 min read Free Webinar | Sept 5: Tips and Tools for Making Progress Toward Important Goals 3-D has come a long way since the days of goofy red-and-blue glasses. You can now buy special computer displays that show 3-D images, and the technology is making inroads on the internet. But is web 3-D ready for its close-up? Tony Parisi, president of Media Machines and the developer of early web 3-D technology VRML, thinks so.What exactly is web 3-D? Parisi, 42, describes it as real-time 3-D graphics that are delivered over a web connection. That’s pretty straightforward. Online gaming is usually the first thing that comes to mind, but the potential uses for the technology go far beyond shoot-’em-ups. “This technology is used for visualization across the board,” says Parisi. That could include an interactive tour through the human body, a virtual tour of a building, or a product catalog that lets customers look at every angle of a piece of equipment and even virtually place it in their office environment to see how it fits before buying. The potential e-commerce applications could be especially intriguing for entrepreneurs in general.Web 3-D has been tried before. Proprietary formats have come and gone, but Parisi sees a future for the current incarnations of open standards like X3D and VRML. “There’s more sizzle to it; the graphics do more and look better,” he says. The times have also changed, making web 3-D’s prospects a lot stronger than in the past. “The base platform is capable now. The network infrastructure is capable now. Broadband is a huge enabler for this. The world is pretty much ready for this,” Parisi says.The next step is widening the deployment of the technology. Early adopters are already using web 3-D, and Parisi estimates more widespread adoption within five years. If the technology catches on with consumers and businesses, it could become as widespread as Flash. Entrepreneurs who work in engineering, tourism, architecture and other highly visual fields may want to look into the possibilities of web 3-D. To see examples of web 3-D at work, visit Media Machines. For the latest news and developments, visit the Web3D Consortium. Attend this free webinar and learn how you can maximize efficiency while getting the most critical things done right. Register Now »
How Success Happens 5 min read Hear from Polar Explorers, ultra marathoners, authors, artists and a range of other unique personalities to better understand the traits that make excellence possible. March 24, 2006 Retail may not be dead, but it is finding new life online.Retail overstock, damaged items and customer returns have longfound buyers on eBay. Now, many retailers are turning to the onlinemarketplace to expand their core business or to become multichannelsellers, without the cost of leasing more real estate.The move from a local retail store to a global online marketwill likely require a shift in sourcing to match demand frommillions of potential new customers. Some retailers simply buy morefrom existing suppliers, especially if bigger orders result in abetter price. But this strategy overlooks the potential of eBay tohelp sellers experiment and diversify, creating a more stablebusiness with a broader customer base.To tap that potential, businesses will need to find newwholesalers, distributors and manufacturers. It’s a processthat can require days of internet searches, but trade shows, onlinewholesale portals and merchandise marts make your job a littleeasier by pulling together names and contact information in onecentral location.Unlike many eBay newcomers, retailers have the advantage ofproven sales performance to help win over vendors wary aboutselling to internet businesses. eBay’s wholesale listingsovercome the problem altogether by linking retailers with sellersalready comfortable with the eBay process.Many businesses turn directly to overseas manufacturers, usuallythrough facilitators or agents with experience in foreign markets.Online agents such as ImportExportHelp.com provide web-based forumsthat list manufacturers looking for buyers.Specialized magazines on sourcing and overseas sourcing fairsare other ways to find manufacturers. You can also look for tradeleads by visiting http://globaledge.msu.edu, a site created by theMichigan State University Center for International BusinessEducation and Research that provides information on 197 countries,a directory of exporting companies from around the world and freeonline training for first-time importers.Jay A. Mednikow (eBay User ID: mednikow), president of J.H.Mednikow & Co. Inc., in Memphis, Tennessee, didn’t chooseany of these options. Instead, he maximized his jewelrybusiness’s unique advantage: customer trade-ins.Trade-ins make up the bulk of Mednikow’s $35,000 monthlyeBay sales, supplemented with consignment items and closeoutmerchandise. The popularity of this refurbished jewelry was sosuccessful, Mednikow, 41, began to use local newspaper advertisingto bring in more trade-in customers. Not only has this line ofbusiness helped build up the company’s eBay volume, but it alsoworks symbiotically when trade-in customers use the cash forsomething new from the Memphis store.Closeouts offer another special advantage, Mednikow says,because after more than five years selling on eBay, he knows how tospot the items that will be most in demand and go for the highestprices. Sources for closeouts and consignments often come fromtrade shows where Mednikow chats with vendors and talks about hissuccess on eBay. “It’s a matter of stepping up thosecasual conversations and making them a little moreaggressive,” he says. “We ask if they have anythingsitting in their vault that they would love to turn intocash.”This enterprising entrepreneur also makes use of the extragemstones he buys when designing new pieces. Mednikow fits thestones, which don’t have much market value on their own, intosimple silver or gold mounts, and those go on eBay, too.”There are endless ways to source product when building abusiness on eBay,” Mednikow says. “It’s an amazingbusiness model.”Work With What You HaveSome retailers are content to stick with what already sells intheir physical stores, using eBay as a sales channel formerchandise that misses the mark with local customers. That’show eBay works for Debbi McGill (eBay User ID:chulavistamojosounds), co-founder of Mojo Sounds, a new and usedCD, DVD and video store in Chula Vista, California. McGill, 39,stocks her music collection with an eclectic mix of bands thatdon’t always appeal to the store’s regular customers. After30 days without a buyer, unbought CDs go on eBay, where they canreach fans from all over the world. “We have bands you’venever heard of that will sell on the internet,” McGill says.The store now has sales between $3,000 to $4,000 a month on eBay,an achievement McGill attributes in part to sales managementsoftware Monsoon Gold.Alan Iba (eBay User ID: iisports), president of I&I SportsSupply Co. Inc. in Carson, California, is another retailer who getsthe most value from selling items related to his core business ofmartial arts, airsoft and paintball supplies-but not the same oneshe puts on the shelf. Local buyers want the latest and greatest,says Iba, 49, while eBay customers mostly look for items theycan’t find anywhere else, including discontinued paintball gunsor collectible martial arts equipment.Supplying these items is a shift for Iba, who originally usedeBay to unload the occasional customer return or damaged goods. In2004, his Certified Provider, Marketworks Inc. in Atlanta,convinced him to launch a full-fledged online business. To supplythe new operation, Iba had to broaden his base of suppliers. Amongthem are nontraditional sources such as martial arts instructorswho have extra tapes from seminars and want to reach followers inother cities. “Small suppliers were always offering product tous,” Iba says, but the company would usually decline. “Wecouldn’t pay top dollar for something that might sit on theshelf,” he explains. But now, Iba says he can offer betterprices that make the deal a win-win for both, because products movefaster online.A variety of strategies help make eBay work for growing retailbusinesses, especially those that know how to maximize the value ofany kind of unsold merchandise. “Someone once told me,”says Mednikow, “that the most successful retailers are thosewho have the best endgame for leftover merchandise.”Julie Monahan is a writer in Seattle whosearticles on small business and emerging technology have appeared innumerous consumer and trade magazines. Opinions expressed by Entrepreneur contributors are their own. Listen Now
Growing a business sometimes requires thinking outside the box. September 20, 2012 5 min read Register Now » Free Webinar | Sept. 9: The Entrepreneur’s Playbook for Going Global When Apple announced the latest version of its popular smartphone, the iPhone 5, CEO Tim Cook proclaimed that their new device was the best iPhone the company has ever made. But is it the best phone on the market right now for business owners?Though it’s a few months old, the Samsung Galaxy S III is still a hot phone thanks to its multi-carrier availability, large and beautiful display, fast 4G LTE capability, and robust performance. The iPhone 5 can boast all of that and more.We’ve done a side-by-side comparison of both phones to determine which one business owners should want to consider. Here’s what we found:Display: Is bigger better?The iPhone 5 finally broke the mold and expanded to 4 inches, up from 3.5. The resolution went up accordingly, and now you can enjoy 1136 x 640 pixels in the Retina display. Compared to many Android phones, including the 4.8-ich Galaxy S III, 4 inches feels a little on the small size these days. That can be a good or bad thing, depending on how much screen you want.For some, 4.8 inches is too big and more difficult to use one-handed. Four inches is stretching it, especially in that department. However, the almost (but not quite) 16:9 aspect ratio means the iPhone 5 is narrow compared to its height. The larger size also means fitting more on the screen, which can be a bonus for busy business owners.The iPhone’s Retina display has a resolution, or pixel density, of 326 pixels per inch versus the GSIII’s 306 ppi density. Technically, the iPhone’s density is better, but not by so much that most people will be able to tell by looking.Related: Top 5 hidden Galaxy S III features you don’t want to missCameras: Front-facing HD, better low-light performanceWhere the iPhone 5 gives the GSIII real competition is with its camera. Apple upped the ante with its rear-facing 8-megapixel camera, adding a dynamic low-light mode and speeding up the picture-taking process. The GSIII’s rear camera (also 8 megapixels) is fine but doesn’t always perform well — especially in low light.In front, the iPhone 5 finally has an HD camera (1.2 megapixels) that can record video up to 720p. This should make FaceTime chats even better and clearer. The Galaxy’s front camera is 1.9 megapixels and also capable of 720p video.Performance: Known unknownsApple claims its new A6 processor chip is twice as powerful as the A5 in the iPhone 4S. That means better, richer gameplay and more complex apps down the line. Meanwhile, the Galaxy S III’s dual-core chip handily beat the iPhone 4S in performance benchmarks. It remains to be seen if the A6 offers as good or better performance.One aspect that could keep the Galaxy on top is that Samsung’s phone has 2GB of RAM. The iPhone 4S had only 512MB when most phones had at least 1GB. Apple doesn’t list it on the Apple website, but the iPhone 5 probably has 1GB.Apple claims that users can expect eight hours of battery life from the iPhone 5, even with the LTE capability, which traditionally draws a lot of power. The GSIII gets between eight and 12 hours depending on the usage and tweaks. Plus, if you need to go a super-long time without charging up, it’s possible to carry and swap out an extra battery.Related: Everything you need to know about the iPhone 5Operating System: iOS 6 vs. Android 4A new iPhone means a new iOS, and version 6 adds a lot of useful features. It also takes away a few things, such as Google Maps and the YouTube app.Even though the iPhone 5’s screen is bigger, apps will still work fine. Until developers update them, older apps will show in a letterbox-esque mode with black bars so you won’t see any stretching or distortion. Apple apps are already optimized for the larger, longer display.Siri also gets an upgrade in this version, which makes her a more useful companion when searching for sports scores or movies. Since the iPhone now has three mics, she’ll be able to understand you better, especially when on the road in noisy areas.The Galaxy S III is still running Android 4.0, which is one version behind the very latest from Google. The upgrade is coming and will bring with it Google Now and other Jelly Bean features. But even with Samsung’s S Voice with the GSIII, it’s often not as useful as Apple’s Siri.Price: Are they worth it?The iPhone 5 starts at $199 for the 16GB version, $299 for 32GB, and $399 for 64GB with a two-year contract.Galaxy S III pricing starts out at $199 for 16GB with a contract on most carriers (T-Mobile costs more), but the carriers offering the 32GB version usually have it at $249, $50 less than the iPhone 5. There’s no 64GB version of the GSIII in the U.S.Bottom line: No clear winnerBoth the iPhone 5 and the Galaxy S III have features that appeal to business owners with a range of mobile needs. Some will prefer the iPhone for the benefits of iOS and the huge app selection, the more compact design, or even better cameras. Others will choose the Samsung’s GSIII for the bigger display, expandable memory, replaceable battery or less restrictive operating system.Related: Samsung Galaxy S III review This story originally appeared on Tecca